![]() ![]() As the rise of the G Corporation brought carnage across the world, a large portion of the Zaibatsu's special military unit, the Tekken Force, defected from the organization. The Mishima Zaibatsu started a war against the world. Even Heihachi was not aware of this piece of information. Only he knew the fact that he drew the Mishima bloodline through his father, Heihachi. Despite this high rank, Lars often fought on the front lines, which made him quite popular among his subordinates. Lars Alexandersson led the coup d'état and became a ranking officer in the Tekken Force at a fairly young age due, in part, to his renowned physical and mental ability. ![]() In the fray, a large portion of the Zaibatsu's special military unit, the Tekken Force, defected from the organization. The rise of the G Corporation opposition resulted in a global trail of carnage. The rebel army Lars leads is named Yggdrasil. Lars's coup d'etat becomes the foundation of a rebellion who's mission is to end the war the Zaibatsu started. ![]() Lars's goal is to bring peace by defeating his half-nephew, Jin Kazama. Most importantly, he is the illegitimate son of Heihachi Mishima, and with the exception of Lars, no one, not even Heihachi, is aware of this piece of information. The mastermind behind this coup d'etat was Lars Alexandersson, a young and charismatic Tekken Force officer with incredible physical ability and intellect. Meanwhile, the Zaibatsu's former Tekken Force soldiers began a coup d'etat against the Mishima Zaibatsu. Outfits Main article: Lars Alexandersson/Outfits Story Tekken 6: Bloodline RebellionĪs the Mishima Zaibatsu waged war on the world, G Corporation, led by Kazuya Mishima, rose from titanic to power and was their main opposition, leading to worldwide conflict. He is usually shown wearing a full military body suit, with armor on some portion of the suit shaped like a lion's head - either Tekken Force armor, or modified into Yggdrasil armor. His facial features bear some resemblance to his father, Heihachi Mishima. Lars has sandy brown hair and blue eyes, with his hair sweeping up on the right side into spikes along the back. Lars is a pragmatic soldier and unafraid to bring a gun to a fistfight if it serves his purpose. Whilst he is a strategist, he also trusts others to lead, and often used to give command of his troops to Tougou so that he himself could fight on the front line. Lars is self reliant, initially treating others with suspicion, as when Lee Chaolan first proposed an alliance with him, but will trust and rely on those who are proven to be loyal to him, such as Tougou, Alisa, and (later) Lee. In Tekken 7, he easily sets aside his previous alliance with Raven in order to recover Jin for his owns ends, and also sets aside his dislike of Jin, whilst persuading the Journalist to do the same, since Jin is necessary for his plans. Lars has been shown both to have an emotional side to him - expressing anger and sorrow in Tekken 6, especially at the loss of his lieutenant, Tougou, but also to have the ability to set aside his personal feelings in order to do what is practical and necessary. He has also been shown to be short tempered as he was quick to draw a gun on his father and shoot him during Tekken 6, and became irate and furious with Jin Kazama when the latter taunted him and set his friend, Alisa Bosconovitch, against him. He is clearly a man of charisma and commands the respect of his peers, as nearly half of Tekken Force chose to join his rebel army during his insurrection. When the Mishima Zaibatsu no longer aligned with what he believed to be right, he led a coup against them. Lars has a heroic personality and a strong sense of justice. 3.3 Naruto Shippūden: Ultimate Ninja Storm 2. ![]()
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Helicon remote wifi canon9/20/2023 ![]() ![]() Authentication – the kind of authentication in the target network, most likely – WPA2. Communication mode – “Infrastructure” for connecting to a Wi-Fi router / access point, “Ad-hoc” for direct connection to a computer’s or laptop’s Wi-Fi adapter. SSID is the name of the network you’re connecting to. – Enter profile name, set connection type to “Computer” and interface type to “Wireless and Ethernet”, press “Next” Otherwise leave the password field blank, or tick “Reset password” and set a desired password – enter a password if it has been already set up previously. Select the “Add new profile” item in the list and press “Next” A new screen “Select a profile” will be shown once the connection is established. – the utility will take some time to connect to the camera. – select “Add / Edit profiles”, press “Next” – select the type of your Wi-Fi unit (WT-4 or WT-5), press “Next” – launch Wireless Transmitter Utility, press “Next” – connect the camera to a computer with USB cable, turn the camera on To do so you will need Nikon Wireless Transmitter Utility. Create a profile for connecting the camera to your network, if you don't have it. Automatic parameters will most likely work, if not – contact your network administrator.ġ. – enter network access key (password), press MENU to confirm ![]() – select “Enter 8-63 ASCII characters”, press ОК Note that WFT unit may not detect networks that occupy wireless channel higher than 7 – if it’s a wireless network – select your network name from the list. – EOS Utility (this is the only mode Helicon Remote will work with) Set Wi-Fi unit up and connect the camera to the network. You should see an "Auto-detected Nikon camera" item. Switch back to Helicon Remote and Press "Select a camera". Then activate Wi-Fi (same menu -> Network connection: ON)Ħ. Clear the Wi-Fi settings in the camera body: menu -> Setup menu -> Wi-Fi -> Network settings -> Reset network settings. ![]() ![]() Make sure no other camera-related apps are runningģ. You should see an "Auto-detected Canon camera" item. Connect your computer or mobile device to the Wi-Fi network created by the cameraĦ. Make sure the camera is turned on and Wi-Fi is active (look for the Wi-Fi icon on the camera body screen)ĥ. Clear the Wi-Fi settings in the camera body, then reactivate Wi-Fi. You may need to uninstall it if you have it (in some cases there's no other way to prevent it from "taking over" control of the camera)ģ. Make sure no other camera-related apps are running, especially Canon Connect. If it doesn't have one, Wi-Fi connection will only be possible with external wireless adapter, like Wu-1a, WT-4a, WT-5A for Nikon cameras, and WFT-4A, WFT-5A, WFT-6A/B, WFT-E7A, WFT-E8A for Canon cameras.įull list of compatible Wi-Fi cameras, both with built-in unit and requiring extenral wirelsess adapter can be found on our website.Ĭonnecting a Canon camera with built-in Wi-Fi unitĬonnecting a Nikon camera with built-in Wi-Fi unit or Wu-1 external unitġ. In order to be able to connect via Wi-Fi, a camera should have a built-in Wi-Fi unit. iOS version of the app supports Wi-Fi connection only, connecting your camera via USB cable is not possible if you use Helicon Remote on an iOS device. I'm about out of troubleshooting ideas.Helicon Focus Help - Helicon Remote Connecting via Wi-Fi: setup guideĪll versions of Helicon Remote - for Android, iOS, Win and Mac OS - allow for wireless connection with your camera. I can also observe outbound TCP traffic from the camera on the correct port, and see an acknowledgments from the PC - but still no joy. When the PC is connected wirelessly, I can ping the camera successfully and have confirmed that the Helicon firewall rules for TCP and UDP are in place and enabled. Helicon Remote works as expected when the camera is connect to the PC via USB. The Canon EOS utility will connect to and control the camera successfully in either type of wireless connection. Adding the camera's IP address manually does not change the symptom. Has anyone tethered a Canon 5D-MK4 to Helicon Remote successfully using the camera's built-in WiFi capability? Helicon Remote will not recognize the camera when connected to the PC in either the Ad-Hoc or Infrastructure wireless modes. ![]() Uriah heep look at yourself9/20/2023 ![]() ![]() ![]() Seeing the writing on the wall, the band opted to pursue the more commercial approach of honing their chops into the less progressive arena rock which proved to be the right move financially speaking however on LOOK AT YOURSELF there are still plenty of progressive rock moments churning about despite no 16-minute closer with orchestral effects in sight. While prog lovers salivated over “Salisbury,” the rock world wasn’t so keen upon this bizarre musical statement so early on and has to be relegated to classic status over time rather than having achieved instant success for URIAH HEEP. He was replaced by Ian Clarke of Cressida who turned out to have the extra drumming fiery passion that was conducive to the harder rocking style the band was settling upon. The result was an album that would steer the course of the band’s sound of permanently settling into the hard rock style that would cement the band’s success beginning with the following “Demons and Wizards.” In between the two albums of 1971, drummer Keith Baker left the band not because of any dramatic altercations with the band or the musical direction but because he didn’t want to endure the extensive tour schedules. On LOOK AT YOURSELF, the band stepped back from the progressive rock influences a bit and went back down the road of hard rock with prog elements. Thanks to Pekka, cannon, Time Signature, Lynx33 for the updates Buy URIAH HEEP - LOOK AT YOURSELF musicĪfter diving first into the possibilities of progressive rock mixed with the heavy blues rock of the 60s on the band’s first album of 1971 on the utterly unique “Salisbury,” URIAH HEEP crafted yet another album the same year. What Should Be Done (live at the BBC) (3:26) Look At Yourself (live at the BBC) (4:32) 14. What Should Be Done (alternate version) (4:26) 13. Tears In My Eyes (extended mix) (5:38) 12. Look At Yourself (single edit) (3:19) 11. What's Within My Heart (out-take) (5:23) 9. What's Within My Heart (out-take) (5:23) Reissued by Castle in 2003 with the following bonus tracks: 8. David Byron / vocals - Ken Hensley / guitar, vocals, piano, organ - Mick Box / guitar - Paul Newton / bass guitar - Ian Clarke / drums, percussion - Manfred Mann / synthesizer (track 3) - Teddy Osei / percussion (track 1) - Mac Tontoh / percussion (track 1) - Loughty Lassisi Amao / percussion (track 1)Ĩ November 1971 Bronze, Mercury Reissued by Castle in 1996 with the following bonus tracks: 8. All in all, Look at Yourself is both one of Uriah Heep's finest, most cohesive albums and a high point of 1970s heavy metal.1. Special note should also be taken of David Byron's vocal performance his multi-octave, operatic style was no doubt an influence on later metal vocalists like Rob Halford. However, the best track on the album is one of the more prog-oriented ones: "July Morning" starts with a pastoral organ riff, then builds into a heavy yet symphonic rock tune that divides its time between gentle acoustic verses and emotional, organ-fueled choruses before climaxing in a monstrous jam dominated by a swirling Moog synthesizer lead. In the rock arena, the gems are "Tears in My Eyes," a powerful rocker driven by an almost rockabilly-style riff that stops midway for a surprising vocal harmony break supported by smooth wah-wah guitar, and "Love Machine," a short, punchy slice of hard rock built on an infectious, stomping rhythm. The remainder of Look at Yourself presents an effective blend of gutsy guitar rock and organ-fueled prog excursions. The gauntlet is thrown down on the title track, a powerful rocker that layers its relentless hard rock attack with ornate vocal harmonies and quicksilver organ runs before climaxing with a tribal-sounding drum jam. The third time proved to be the charm for Uriah Heep: on Look at Yourself, the group perfects its fusion of heavy metal power and prog rock majesty, and the result is one of the best albums in the Heep catalog. ![]() Julian steward9/20/2023 ![]() ![]() Steward’s theory centered around a culture core, which he defined as “the constellation of features which are most closely related to subsistence activities and economic arrangements” (Steward 1955:37).īy the 1960s and 1970s, cultural ecology and environmental determinism lost favor within anthropology. He looked for the adaptive responses to similar environments that gave rise to cross-cultural similarities (Netting 1996:267). The detailed ethnographic accounts of Boas, Malinowski, and others led to the realization that environmental determinism could not sufficiently account for observed realities, and a weaker form of determinism began to emerge (Milton 1997).Īt this time, Julian Steward coined the term “ cultural ecology” (see Principal Concepts). The earliest attempts at environmental determinism mapped cultural features of human populations according to environmental information (for example, correlations were drawn between natural features and human technologies) (Milton 1997). Therefore, an ecosystem (see Principal Concepts) consists of organisms acting in a bounded environment.Īs a reaction to Darwin’s theory, some anthropologists eventually turned to environmental determinism (see Principal Concepts) as a mechanism for explanation. This includes the relationships of animals with the inorganic and organic environments, above all the beneficial and inimical relations Darwin referred to as the conditions for the struggle of existence” (Netting 1977:1). Haekel coined our modern understanding of ecology in 1870, defining it as “the study of the economy, of the household, of animal organisms. The word “ ecology” is derived from the Greek oikos, meaning habitation. This circumstance leads to disease and hunger which eventually put a limit on the growth of the population (Seymour-Smith 1986:87). Malthus pioneered demographic studies, arguing that human populations naturally tend to outstrip their food supply (Seymour-Smith 1986:87). Malthus (see Leading Figures) had an obvious influence on Darwin’s formulations. ![]() It is the environmental context that determines whether or not a trait is beneficial. Individuals with favorable characteristics, or variations, survive to reproduce. In each generation, more individuals are produced than can survive (because of limited resources), and competition between individuals arises. In The Origin of Species (1859), Charles Darwin presented a synthetic theory of evolution based on the idea of descent with modification. In a general sense, ecological anthropology attempts to provide a materialist explanation of human society and culture as products of adaptation to given environmental conditions (Seymour-Smith 1986:62). ![]() Ecological anthropology investigates the ways that a population shapes its environment and the subsequent manners in which these relations form the population’s social, economic, and political life (Salzman and Attwood 1996:169). ![]() Human populations have ongoing contact with and impact upon the land, climate, plant, and animal species in their vicinities, and these elements of their environment have reciprocal impacts on humans (Salzman and Attwood 1996:169). Ecological anthropology focuses upon the complex relations between people and their environment. ![]() Macro recorder9/20/2023 ![]() ![]() You use the Visual Basic Editor to edit the macros attached to a workbook. There are several different ways you can run a macro, such as using a shortcut key, graphic object, Quick Access Toolbar, a button, or even when opening a workbook. Read specific information on available security settings for macros, and their meaning. Here's further information on working with macros in Excel. If you run a macro and it doesn’t do what you want, you can close the workbook without saving it. Before you run a recorded macro for the first time, make sure that you’ve either saved the workbook where you want to run the macro, or better yet work on a copy of the workbook to prevent unwanted changes. This opens the Macro dialog box.Ĭaution: Macros cannot be undone. In the Developer tab, click Macros to view macros associated to a workbook. On the Developer tab, in the Code group, click Stop Recording. Perform the actions that you want to record. If you create a lot of macros, the description can help you quickly identify which macro does what, otherwise you might have to guess. Also, try to enter a meaningful description with any information that may be useful to you or other users who will be running the macro. In the Description box, optionally type a brief description of what the macro does.Īlthough the description field is optional, it is recommended you enter one. When you select Personal Macro Workbook, Excel creates a hidden personal macro workbook (Personal.xlsb) if it does not already exist, and saves the macro in this workbook. In general, you’ll save your macro in the This Workbook location, but if you want a macro to be available whenever you use Excel, select Personal Macro Workbook. In the Store macro in list, select where you want to store the macro. For instance, if you use Ctrl+Z (Undo), you will lose the ability to Undo in that Excel instance. It is best to use Ctrl + Shift (uppercase) key combinations, because the macro shortcut key will override any equivalent default Excel shortcut key while the workbook that contains the macro is open. To assign a keyboard shortcut to run the macro, in the Shortcut key box, type any letter (both uppercase or lowercase will work) that you want to use. If you use a macro name that is also a cell reference, you may get an error message that the macro name is not valid. Spaces cannot be used in a macro name an underscore character works well as a word separator. ![]() Subsequent characters can be letters, numbers, or underscore characters. Note: The first character of the macro name must be a letter. Make the name as descriptive as possible so you can quickly find it if you create more than one macro. In the Macro name box, enter a name for the macro. On the Developer tab, in the Code group, click Record Macro. For example, you can record a macro where you first update a table in Excel and then open Outlook to email the table to an email address. ![]() Your macro process can extend to other Office applications, and any other applications that support Visual Basic Application (VBA). It is not necessary that only tasks in Excel can be recorded in a macro. If you have planned a long process of tasks to record, plan to have smaller relevant macros instead of having one long macro. So if you added an extra row to the range, the macro will not run the process on the new row, but only the cells within the range. When you record a macro for performing a set of tasks in a range in Excel, the macro will only run on the cells within the range. There are a few helpful things you should know about macros: For more information, see Show the Developer tab. Macros and VBA tools can be found on the Developer tab, which is hidden by default, so the first step is to enable it. ![]() The more smoothly you record a sequence, the more efficiently the macro will run when you play it back. This is why whenever you record something, it's best to record a process with which you're highly familiar. The resolution is to re-record the entire sequence, or modify the VBA code itself. So if you make a mistake in your sequence, for example, clicking a button that you did not intend to click, the Macro Recorder will record it. It is important to know that you when you record a macro, the Macro Recorder captures almost every move you make. Although VBA gives you the ability to automate processes within and between Office applications, it is not necessary to know VBA code or computer programming if the Macro Recorder does what you want. Visual Basic Application (VBA) is a subset of the powerful Visual Basic programming language, and is included with most Office applications. These steps can include typing text or numbers, clicking cells or commands on the ribbon or on menus, formatting cells, rows, or columns, or even importing data from an external source, say, Microsoft Access. When you record a macro, the macro recorder records all the steps in Visual Basic for Applications (VBA) code. ![]() Flatout bears9/20/2023 ![]() ![]() Designed to act as a natural comforter, this bear is suitable for use from birth and complies with all relevant EU safety regulations. ![]() In a situation where we feel the look and style of flowers would be too different to your original selection we will contact you to determine how you would like us to proceed. Cuddly and super soft with a cute face, this bear is crafted in pure shearling with a baby-safe shorter pile and a simple, flat shape that little hands will enjoy fiddling with and heads will love resting on. Any substitution will be made in keeping to the design of your selection. A unique product used as a babys toy and comforter. ![]() FLATOUT Baby Bears are super adorable and the must have baby item. Hugh Jackman and Deborra-Lee Furness, Jennifer Garner,Sarah-Jessica Parker. As such, there will be times when substitute flowers (of equal or greater value) may need to be used. Soft, cuddly and flat, the FLATOUTbear is made of 100 pure sheepskin. Fabulous and famous, FLATOUT Bears have become an Australian Icon. And it seems that celebrities especiallylove them. Since the COVID-19 pandemic this has become more common as the regular supply and availability of specific flowers and varieties has become less certain. Each sheepskin varies which makes each FLATOUT bear unique and special. However, there may be times when specific varieties will be unavailable due to seasonal factors as well as occasions when we deem the quality of the available supply is diminished and not suitable for our clients. FLATOUT bears are made of 100 Australian sheepskin. Otherwise the flowers, allowing for Mother Nature, should be the same. As you would expect, they, will be bigger or smaller if a smaller or larger size than what is shown is purchased. Please note, our aim is to supply flowers as per the image shown above. ![]() Tableflip emoji9/20/2023 ![]() What are the steps needed to create an ad?īefore you create publicity, there are a few things to bear in mind. Interactive advertising uses engaging media, whether online or offline, to connect with customers and support commercials, goods, brands, services, and public services.ģ. The creation and arrangement of visual artwork used to promote and market products. If you want to create effective and captivating advertisements for your brand, get started with Glorify today! FAQsġ. These include an instant background remover, a logo maker, Brand Kits, Smart Shapes, an advanced pen tool, template bundles, mockups and a whole lot more! In addition, there are a ton of other useful and fun features that can boost your ad design game. It provides thousands of niche-based templates that are 100% editable and can be customized to suit your brand. If ad design is what you're into right now, Glorify's template library is definitely something you should check out. ![]() With simple tools for beginners and complex features for individuals, Glorify allows anyone and everyone to create stunning visuals regardless of prior design experience. You can try your hand at ad design by starting of with an easy-to-use design tool such as Glorify. However, while it certainly helps to produce a visually effective advertisement that plays by the rules, the success of an ad comes down to artistic thought and the drive to be unique. Like in any other marketing asset, there are comprehensive rules, strategies, procedures, and techniques for ad production. ![]() Freeplane tutorial on yiutube9/20/2023 ![]() ![]() Groovy scripts can be run on Freeplane interface, which allows automation. A map can be presented as a slide presentation for students. A structured text document or note can be converted to map or vice versa. Teachers can share the learning materials, as a map or in other formats. They can be tailor made to the level of learner’s understanding. Icons and formatting can make the nodes visually stand out in a map. Learning resources such as notes, text, image, web page hyperlink, audio or video can be attached to nodes. Properties of Each node can be formatted as desired. It is a useful software for brain storming. Ideas and concepts can be arranged hierarchically or otherwise and can be connected with a line. The visual representation helps to bring about better understanding and learning. ![]() In a concept map, the ideas or concept is presented visually in a connected fashion with fewer words. Teachers can use it to teach and students can use it for structured learning. Concept mapping or Mind mapping was developed by Prof. Thanks to its intuitive layout yet rich options, Freeplane should be able to please the entire audience.Freeplane is a mind mapping or concept mapping open source software, and is actively developed. No error dialogs have been shown throughout our tests and the app did not hang or crash. It has a good response time and includes user documentation. The comprehensive program requires a pretty high amount of CPU and system memory to work properly. and tutorial and documentation in the form of a mind map.
Untitled goose game toothbrush9/20/2023 ![]() ![]() She’ll have to escape via the gate, which will open to the next area: Back Gardens.īack Gardens - Untitled Goose Game Walkthrough Lure her in with something, and pull the string when she’s in. The final To-Do item requires trapping the shop owner in the garage. While playing this game of tug-o-war, the broom will eventually break. To break the broom, you have to grab it and allow the shop owner to try and wrestle it away. Tip over the trash can to snag the toothbrush. The cleaner and toothbrush are nearby the trash can that is on the eastern side. The loo paper, hairbrush, tinned food, and fruit & veg are all found within the shop stands. If done successfully, he’ll have to buy back his plane from the shop owner.Īs for the shopping items, I recommend dragging the nearby basket farther away to prevent it being seen by the shop owner. You might have to lure him with the toy plane first, and then drop it within the shop area before the boy can claim it. This is the tricky bit, though - you’ll need to get the boy’s attention over to the shop area to find his plane. The shop owner will pick it up and place it along with the others. Drop this toy nearby the shop stand with the other toys. To further harass the poor boy, you can take his blue plane that is placed on the bench. Goose can hide under the table and watch. The boy will stand here to buy back his blue plane. Move around inside the shop to get on T.V. shop owner to come out to help the boy, then run in to press the red switch. He’ll run inside, and use the phone to call the T.V. Then honk at him, leading him towards the red phone booth in the northwest corner. Place the glasses near the boy, and he’ll wear them. Be careful though, because the shop owner does not welcome goose in that area. You can find replacement glasses in the shop/stand area. You can trip him by pulling at his shoelaces, causing him to fall. go shopping (put in basket: toothbrush, loo paper, hairbrush, tinned food, cleaner, fruit & veg).High Streets - Untitled Goose Game Walkthrough The gateway will open to the next area: High Streets. The final To-Do item requires honking while the groundskeeper is hammering the sign. Immediately stealth-run into the tall brush garden to the eastern side and hide. He’ll kneel down and re-plant it, allow you to snatch his hat. To get the groundskeeper to wear his sun hat, grab the single red flower but allow the groundskeeper to return it. The rake can be found in the far end of the garden, and you’ll need to drag that all the way to the lake. He will find all the items and begin putting them all back one-by-one. Make sure to give a wide berth to the groundskeeper, and don’t lead him back to your picnic blanket. The other picnic items can be found in here: the pumpkin, carrot, thermos, and jam. Or you can leave something by the sprinkler system, and then turn it on. If you head towards the lake, he’ll chase you, and you can get him wet in that manner. The groundskeeper will then unlock the gate. To get into the garden area, you can turn on the sprinkler system. After stealing his hat, head directly into the bushes to escape the groundskeeper. You can swim with it into lake to shut it off, to prevent the groundskeeper from being alerted. The radio is located atop the fertilizer bags near the gate into the garden. The bench nearby has the basket, sandwich (both halves), and apple. You can find the red picnic blanket to the east, near the lake’s edge. This is where the garden is located - and where the groundskeeper works. The main direction you’ll want to focus on is in the northeast. Have a picnic (bring to the picnic blanket: sandwich, apple, pumpkin, carrot, jam, thermos, radio, basket). ![]()
Smartscope fold microscope9/20/2023 ![]() ![]() They just involve different types of signals. The engineering and computer science needed to analyze stock market data and those needed to analyze biological data are not dramatically different. What does a real neural network look like, and what do we need to help us decode it? on artificial neural networks, so it was very natural for me to ask questions about real biology, actual brains. I’ve been working on biology and biomedicine-related problems from the very beginning. How did you get interested in analyzing biological image data? Why not analyze data from, say, the stock market? This new system, called Vaa3D, has attracted tens of thousands of downloads from all over the world. Recently, I started to combine the tools from my labs with some other tools contributed by my colleagues into a new visualization and analysis platform to analyze a diversity of large 3D images. We don’t just develop new tools for specialists in certain areas of biology. For instance, we could apply similar techniques to the mouse brain and even the mouse lung. The tools we develop are useful for more than one particular problem. We designed computer programs to align images and identify and trace neurons. We started building a 3D digital map of the entire fruit fly brain, generating a huge amount of 3D image data and then doing the association image mining. These projects got us interested in the fruit fly’s nervous system, where cells’ identities are well-defined and where we could study how neurons are connected throughout the brain. We participated in several projects to measure gene expression in the fruit fly embryo and C. The data sets were mostly two-dimensional, and we realized that we really needed 3D data for gene expression. How have you applied your image analysis tools?īefore I arrived at Janelia, I worked on mapping gene expression in the fruit fly embryo. Then we produce quantitative measurements for the objects in the image to compare their various dimensions and look for patterns. We normalize objects so that they can be compared to each other directly. How do you compare their intrinsic features? You might compare the size of the head or their body with respect to their overall height. First, we standardize the data into a space so that we can directly compare all these objects. Once we have the objects, we need to find associations among the objects-for example, which neuron connects to which other neurons. How do they define a cell, a neuron? How do they describe the cell population? We use their definitions to train our computer to recognize objects and help scientists do this large-scale, complicated job automatically. We start there and consider the biologists’ knowledge. We call this automated process “image mining.”Ĭan you walk us through how you sift through the data?įirst, you need to define the meaningful objects or patterns. In 3D microscopy, we let the computer pick out interesting objects, like particular neurons, and find the association between the cells, such as the potential connectivity between neurons. It’s not just doing it faster we have to come up with whole new ways to process data that are meaningful for biology. ![]() Therefore, we need to design a smarter way to deal with those data. Confocal microscopes, for instance, can produce images with 1,000 or 10,000 times more pixels than an ordinary picture taken with an ordinary camera.ĭata sets are often so large, and the incoming speed of new data is so much faster than the processing speed, that you’ll never be able to process the data manually. With a 3D image, you’re going to have a lot of data. In the last 20 years, scientists and engineers have come up with super-powerful imaging systems to visualize the three-dimensional (3D) structure of a sample. How do you help biologists make sense of images? ![]() Peng’s toolkit might someday even help the Federal Reserve. Right now, his focus is the brain’s wiring. Now, at the Janelia Farm Research Campus, he is a leader in developing sophisticated ways to make sense of biological images. Even when he launched his career as an engineer and computer scientist, Hanchuan Peng was drawn to the beauty of biology. ![]() AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |